- Title
- Genome-wide mRNA and miRNA analysis of peripheral blood mononuclear cells (PBMC) reveals different miRNAs regulating HIV/HCV co-infection
- Creator
- Gupta, P.; Liu, B.; Wu, J. Q.; Soriano, V.; Vispo, E.; Carroll, A. P.; Goldie, B. J.; Cairns, M. J.; Saksena, N. K.
- Relation
- NHMRC
- Relation
- Virology Vol. 450-451, p. 336-349
- Publisher Link
- http://dx.doi.org/10.1016/j.virol.2013.12.026
- Publisher
- Academic Press
- Resource Type
- journal article
- Date
- 2014
- Description
- Co-infection with human immunodeficiency virus (HIV) and hepatitis C virus (HCV) is common due to shared transmission routes. The genomic basis of HIV/HCV co-infection and its regulation by microRNA (miRNA) is unknown. Therefore, our objective was to investigate genome-wide mRNA expression and its regulation by miRNA in primary PBMCs derived from 27 patients (5 HCV - mono-infected, 5 HIV-mono-infected, 12 HCV/HIV co-infected, and 5 healthy controls). This revealed 27 miRNAs and 476 mRNAs as differentially expressed (DE) in HCV/HIV co-infection when compared to controls (adj p<0.05). Our study shows the first evidence of miRNAs specific for co-infection, several of which are correlated with key gene targets demonstrating functional relationships to pathways in cancer, immune-function, and metabolism. Notable was the up regulation of HCV-specific miR-122 in co-infection (FC>50, p=4.02E-06), which may have clinical/biological implications.
- Subject
- HIV; HCV; co-infection; miRNA; microarray
- Identifier
- http://hdl.handle.net/1959.13/1304880
- Identifier
- uon:20947
- Identifier
- ISSN:0042-6822
- Language
- eng
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